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Journal: Frontiers in Immunology
Article Title: Polymorphisms in STING Affect Human Innate Immune Responses to Poxviruses
doi: 10.3389/fimmu.2020.567348
Figure Lengend Snippet: IFNβ and ISG-56K Promoter Activity is Greater in HEK 293T Cells Stably Expressing the R232 STING Variant. (A) IFNβ promoter induction following cGAMP stimulation. (B) ISG-56K promoter activity after cGAMP stimulation. (C) ISG-56K promoter activity after stimulation with inactivated vaccinia virus (inact. VV). Data points for (A) , (B, C) show the means with error bars representing the standard deviations of three replicates. (D) STING expression in HEK293T stably expressing R232 or H232 STING. Data points for (D) show the means with error bars representing the standard deviations of eight replicates. A two-tailed t-test* detected significant differences; see text for p-values. Each experiment was performed twice with nearly identical results.
Article Snippet: We used pNiFty2-IFNB-SEAP and
Techniques: Activity Assay, Stable Transfection, Expressing, Variant Assay, Virus, Two Tailed Test